资源 - 动态摘要
Quantifying Poly-Glutamine Aggregates in C. Elegans Using vivoChip
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August 24, 2018
C. elegans has emerged as a tool for whole organism based high-throughput screening as they model complex human diseases that can not be easily reproduced in vitro. Here, we use a model of Huntington’s disease which consists of poly-glutamine aggregation (PolyQ35:YFP). C. elegans were loaded onto the vivoChip (Newormics) and imaged in the YFP channel. Outlines of the worms were identified using Gen5, and the secondary mask function was used to count the aggregates per worm.