Resources
应用指南
应用简讯
- BioTek Resources for Agilent MitoXpress® Intra Intracellular Oxygen Assay (January 29, 2019)
- BioTek Resources for Agilent pH-Xtra™ Glycolysis Assay (June 27, 2018)
- BioTek Resources for Agilent MitoXpress® Xtra Oxygen Consumption Assay (June 27, 2018)
- Quantification of GFP Transfection using a Cost-effective Microplate Reader (June 1, 2018)
应用手册
- Nuclear Translocation of RelA in Stimulated Macrophages (September 18, 2019)
- Automated Analysis of Neutrophil NETosis Activity (April 30, 2019)
- Determination of Cell Cycle Time (December 6, 2018)
- Advanced Wound Healing Assay Workflow using Automated Scratch Wound Creation, High Contrast Brightfield and Fluorescence Kinetic Imaging (December 6, 2018)
- XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System (September 5, 2018)
- Stimulation of IL-2 Secretion in Human Lymphocytes (March 21, 2018)
- Brightfield and Fluorescence Imaging using 3D PrimeSurface® Ultra-Low Attachment Microplates (December 21, 2017)
- Automated Imaging and Dual-Mask Spot Counting of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis (December 19, 2017)
- An Image-Based Method to Detect and Quantify T Cell Mediated Cytotoxicity of 2D and 3D Target Cell Models (December 13, 2017)
- Rapid Measurement of IgG Using Fluorescence Polarization (September 15, 2017)
- Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity Assay (March 24, 2017)
- Protein Quantitation using NanoOrange® Fluorescence (January 26, 2017)
- Fluorometric Quantitation of dsDNA using PicoGreen® (January 25, 2017)
- Kinetic Proliferation Assay Using Label-Free Cell Counting (January 25, 2017)
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids (December 16, 2016)
- Automation and Image-Based Analysis of a Hanging Drop Micro-Hole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation (October 25, 2016)
- Utilizing Automated Imaging and Advanced 3D Cell Culture Techniques to Quantify Apoptosis Activity (April 5, 2016)
- Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential Neurotoxicants (March 25, 2016)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (March 21, 2016)
- Comparative Analysis of Dual-Luciferase® Assay Technologies in a High Throughput Microplate Format (March 18, 2016)
- Detection and Visual Localization of Individual Cellular Proteins Using a Proximity Ligation Assay (March 4, 2016)
- High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model (February 18, 2016)
- Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging (February 18, 2016)
- High Content RNAi Screening Using Printed Libraries (February 9, 2016)
- Validation of High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging (February 8, 2016)
- Automated Imaging and Analysis of a Novel Comet Assay to Enable High Throughput Genotoxicity Testing (December 1, 2015)
- Z-Stacking and Z-Projection using a Scaffold-based 3D Cell Culture (May 7, 2015)
- Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses - Comparison of Results Generated with an in vitro Cell Signaling Assay using Cells Cultured in 2D and a Novel 3D Cell Culture System (April 30, 2015)
- Automation of HTRF® elF4E Kinase Assay Using a 3D Tumoroid- Based Cell Model (April 30, 2015)
- Combining Kinetic Ligand Binding and 3D Tumor Invasion Technologies to Assess Drug Residence Time and Anti-metastatic Effects of CXCR4 Inhibitors (April 16, 2015)
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy (April 10, 2015)
- Quantification of MMP Activity and Inhibition in a 3D Tumor Invasion Model (April 7, 2015)
- Analysis of α-Synuclein Fibril Formation in vitro (December 10, 2014)
- Z-Stacking of Single Plane Digital Widefield Fluorescent Images (October 31, 2014)
- Cell Synchrony Determination Using Microscopic Imaging - Use of Nuclear Staining to Assess Cell Cycle Stage by Nuclear DNA Content (October 14, 2014)
- UV Fluorescence Polarization as a Means to Investigate Protein Conformational and Mass Change - Using Intrinsic Tryptophan Fluorescence in Conjunction with UV-capable Polarizers (October 13, 2014)
- Automated, Kinetic Imaging of Cell Migration and Invasion Assays using Corning FluoroBlok™ Permeable Supports (October 9, 2014)
- Inhibition of Hypoxic Tumor Cells using a Three-Dimensional Spheroid Model (August 6, 2014)
- Live Cell Hypoxia Determination in a 3D Spheroid Model (August 5, 2014)
- High Resolution Fluorescence Microscopy of PtK2 Cells Undergoing Mitosis in Microplates (June 24, 2014)
- Live Cell Imaging of RNA Expression (April 18, 2014)
- Automated Cell Dispensing and Image-Based Spheroid Formation Tracking (April 15, 2014)
- Live Cell Imaging of GPCR Dependent Second-Messenger Systems (April 14, 2014)
- The Impact of a 3-Dimensional Human Liver Microtissue Model on Long-term Hepatotoxicity Studies (March 31, 2014)
- Incorporation of a Novel 3D Cell Culture System to Perform in vitro Cytotoxicity Analyses using Human Primary Hepatocytes (March 20, 2014)
- The Effect of Cell Culture Method on Long-Term Primary Hepatocyte Cell Health (February 19, 2014)
- Investigation of Cell Migration using a High Density Cell Exclusion Assay and Automated Microplate Imager (July 10, 2013)
- Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes (June 13, 2013)
- Imaging of BacMam Transfected U-2 OS Cells (April 15, 2013)
- Analysis of Nuclear Stained Cells - Using the Cytation™3 Cell Imaging Multi-Mode Microplate Reader with DAPI-Stained Cells (April 15, 2013)
- Automated Tissue Culture Cell Fixation and Staining in Microplates (April 15, 2013)
- Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Cell Line (April 15, 2013)
引用文献
细胞成像&显微镜
Cytation 1 Cell Imaging Multi-Mode Reader (29)
- Timilsina, U;Umthong, S;Lynch, B;Stablewski, A;Stavrou, S; (2020) "SERINC5 potently restricts retrovirus infection in vivo." bioRxiv
- Miller, J;Meki, M;Ou, Q;George, S;Gams, A;Abouleisa, R;Tang, X;Ahern, B;Giridharan, G;El-Baz, A;Hill, B;Satin, J;Conklin, D;Moslehi, J;Bolli, R;Ribeiro, A;Efimov, I;Mohamed., T; (2020) "Heart Slice Culture System Reliably Demonstrates Clinical Drug-Related Cardiotoxicity." bioRxiv
- Kozisek, T;Hamann, A;Nguyen, A;Miller, M;Plautz, S;Pannier, AK; (2020) "High-throughput screening of clinically approved drugs that prime nonviral gene delivery to human Mesenchymal stem cells." J Biol Eng
- Cassidy, FC;Shortiss, C;Murphy, CG;Kearns, SR;Curtin, W;De Buitléir, C;O'Brien, T;Coleman, CM; (2020) "Impact of Type 2 Diabetes Mellitus on Human Bone Marrow Stromal Cell Number and Phenotypic Characteristics." Int J Mol Sci
- Das, A;Gangarde, YM;Tomar, V;Shinde, O;Upadhyay, T;Alam, S;Ghosh, S;Chaudhary, V;Saraogi, I; (2020) "Small-Molecule Inhibitor Prevents Insulin Fibrillogenesis and Preserves Activity." Mol. Pharm.
- Dutta, P;Paico, K;Gomez, G;Wu, Y;Vadgama, JV; (2020) "Transcriptional Regulation of CCL2 by PARP1 Is a Driver for Invasiveness in Breast Cancer." Cancers (Basel)
- Han, Y;Zhou, CM;Shen, H;Tan, J;Dong, Q;Zhang, L;McGowan, SJ;Zhao, J;Sowa, GA;Kang, JD;Niedernhofer, LJ;Robbins, PD;Vo, NN; (2020) "Attenuation of ataxia telangiectasia mutated signalling mitigates age-associated intervertebral disc degeneration." Aging Cell
- Lawrence, M;Shell, S;Beckford, F; (2020) "Binuclear manganese-iron complexes containing ferrocenyl thiosemicarbazones: biological activity and carbon monoxide-releasing properties." Inorganica Chimica Acta
- Guo, J;Jiang, W;Li, Q;Jaiswal, M;Guo, Z; (2020) "Comparative immunological studies of tumor-associated Lewis X, Lewis Y, and KH-1 antigens." Carbohydrate Research
- Hamann, A;Thomas, AK;Kozisek, T;Farris, E;Lück, S;Zhang, Y;Pannier, AK; (2020) "Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection." Exp. Biol. Med. (Maywood)
- Song, Y;Wang, H;Zhang, L;Lai, B;Liu, K;Tan, M; (2020) "Protein corona formation of human serum albumin with carbon quantum dots from roast salmon." Food Funct
- Milewska, M;Burdzińska, A;Zielniok, K;Siennicka, K;Struzik, S;Zielenkiewicz, P;Pączek, L; (2020) "Copper Does Not Induce Tenogenic Differentiation but Promotes Migration and Increases Lysyl Oxidase Activity in Adipose-Derived Mesenchymal Stromal Cells." Stem Cells Int
- Cladis, DP;Li, S;Reddivari, L;Cox, A;Ferruzzi, MG;Weaver, CM; (2020) "A 90 day oral toxicity study of blueberry polyphenols in ovariectomized sprague-dawley rats." Food Chem. Toxicol.
- Sheng, L;Zhang, Z;Sun, G;Wang, L; (2020) "Light-driven antimicrobial activities of vitamin K3 against Listeria monocytogenes, Escherichia coli O157:H7 and Salmonella Enteritidis." Food Control
- Sheng, L;Zhang, Z;Sun, G;Wang, L; (2020) "Light-driven antimicrobial activities of vitamin K3 against Listeria monocytogenes, Escherichia coli O157: H7 and Salmonella Enteritidis." Food Control
- Li, R;Hornberger, K;Dutton, JR;Hubel, A; (2020) "Cryopreservation of Human iPS Cell Aggregates in a DMSO-Free Solution-An Optimization and Comparative Study." Front Bioeng Biotechnol
- Wentzel, AS;Janssen, JJE;de Boer, VCJ;van Veen, WG;Forlenza, M;Wiegertjes, GF; (2020) "Fish Macrophages Show Distinct Metabolic Signatures Upon Polarization." Front Immunol
- Webb, LM;Sengupta, S;Edell, C;Piedra-Quintero, ZL;Amici, SA;Miranda, JN;Bevins, M;Kennemer, A;Laliotis, G;Tsichlis, PN;Guerau-de-Arellano, M; (2020) "Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity." J. Clin. Invest.
- Kojima, Y;Machida, Y;Palani, S;Caulfield, TR;Radisky, ES;Kaufmann, SH;Machida, YJ; (2020) "FAM111A protects replication forks from protein obstacles via its trypsin-like domain." Nat Commun
- Fessler, E;Eckl, EM;Schmitt, S;Mancilla, IA;Meyer-Bender, MF;Hanf, M;Philippou-Massier, J;Krebs, S;Zischka, H;Jae, LT; (2020) "A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol." Nature
- Genty, C;McDonnell, TJ; (2019) "Flex-Array® - a novel multi-well vessel system for the immobilization and multi-modal testing of intact formalin-fixed paraffin-embedded (FFPE) cells or tissues." J Histotechnol
- Tanuja Koppal (2020) "Innovations in Cell Counting." Biocompare
- Daniels, H;Fast, O;Shell, S;Beckford, F; (2019) "Chemistry and biology of manganese carbon-releasing molecules containing thiosemicarbazone ligands." Journal of Photochemistry and Photobiology A: Chemistry
- Romereim, SM;Johnston, CA;Redwine, AL;Wachs, RA; (2019) "Development of an in vitro intervertebral disc innervation model to screen neuroinhibitory biomaterials." J. Orthop. Res.
- Feng, LR;Wolff, BS;Liwang, J;Regan, JM;Alshawi, S;Raheem, S;Saligan, LN; (2019) "Cancer‑related fatigue during combined treatment of androgen deprivation therapy and radiotherapy is associated with mitochondrial dysfunction." Int. J. Mol. Med.
- Salewskij, K;Rieger, B;Hager, F;Arroum, T;Duwe, P;Villalta, J;Colgiati, S;Richter, CP;Psathaki, OE;Enriquez, JA;Dellmann, T;Busch, KB; (2020) "The spatio-temporal organization of mitochondrial F1FO ATP synthase in cristae depends on its activity mode." Biochim Biophys Acta Bioenerg
- Kam, A;Loo, S;Fan, JS;Sze, SK;Yang, D;Tam, JP; (2019) "Roseltide rT7 is a disulfide-rich, anionic, and cell-penetrating peptide that inhibits proteasomal degradation." J. Biol. Chem.
- Ko, Y;Kim, J;Jeong, HY;Kwon, G;Kim, D;Ku, M;Yang, J;Yamauchi, Y;Kim, HY;Lee, C;You, J; (2019) "Antibacterial poly (3,4-ethylenedioxythiophene):poly(styrene-sulfonate)/agarose nanocomposite hydrogels with thermo-processability and self-healing." Carbohydr Polym
- Liu, T;Zhou, L;Yang, K;Iwasawa, K;Kadekaro, AL;Takebe, T;Andl, T;Zhang, Y; (2019) "The β-catenin/YAP signaling axis is a key regulator of melanoma-associated fibroblasts." Signal Transduct Target Ther
科学期刊
商务期刊
American Laboratory (1)
Genetic Engineering & Biotechnology News (3)
- Joe Clayton, Ph.D. (2017-08-01) "Monitoring the Proliferation of Cells Is Useful to Evaluate Drug-Dose Response." GEN
- Brad Larson (2015-09-15) "Analysis of 3D Cell Culture Models - Enabling Phenotypic and Target-Based Assay of 3D Cellular Structures." GEN
- Peter Banks, Ph.D. (2013-10-1) "PMT-Based Hit Picking for HCA." GEN
Lab Manager (2)
科学海报
- Consistent, Repeatable Wound Healing Assays Incorporating a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging (February 6, 2019)
- Advanced 2D Wound Healing Assay Procedures Performed using a Novel, Automated Scratch Tool, High Contrast Brightfield and Fluorescence Kinetic Imaging (February 6, 2019)
- Investigation of Cell Migration in a Label-free Cell Exclusion Assay by Automated Microplate Imaging (February 6, 2019)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (February 4, 2016)
- Validation of a High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging (February 4, 2016)
- Combining a Novel Comet Assay Procedure with Automated Imaging and Analysis to Enable High Throughput Genotoxicity Testing (February 4, 2016)
- Validation of Th17 Cell Differentiation from Peripheral Blood CD4+ Cells through Assessment of mRNA (February 3, 2016)
- Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging (February 3, 2016)
- Quantification of MMP Activity and Inhibition in a 3D Tumor Invasion Model (September 29, 2015)
- Neurosphere Formation, Migration, and Differentiation of Human Neural Stem Cells Cultured in Corning® Spheroid Microplates (September 21, 2015)
- Improving Fluorescence-Based Assay Performance Using Automated Digital Microscopy (February 13, 2015)
- Automated Cell Dispensing and Image-Based Spheroid Formation Tracking (February 13, 2015)
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy (February 13, 2015)
- Automated, Kinetic Imaging of Cell Migration and Invasion Assays using Corning® FluoroBlok™ Inserts (February 13, 2015)
- Combining Kinetic Ligand Binding and 3D Tumor Invasion Technologies to Assess Drug Residence Time and anti-Metastatic Effects of CXCR4 Inhibitors (February 13, 2015)
- Automated Cell Synchrony Determination Using Microscopic Imaging in Live Cells (February 13, 2015)
- Inhibition of Hypoxic Tumor Cells using a Three-Dimensional Spheroid Model (February 13, 2015)
- Optimization of a multi-mode detection model for measuring real-time cellular respiration and mitochrondrial function using fluorophoric biosensors (February 13, 2015)
- Validation of a 3-Dimensional Human Liver Microtissue Model for Long-term Hepatotoxicity Studies (September 26, 2014)
- Automated Fluorescence Detection and Imaging of RNA Species in Live Cells (September 26, 2014)
- Improving Fluorescence-Based Assay Performance Using Automated Digital Microscopy (September 2, 2014)
- Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses (January 21, 2014)
- Automated Fluorescence Detection and Imaging of RNA Species in Live Cells (January 21, 2014)
- Validation of a Novel 3D Cell Culture System to Perform in vitro Cytotoxicity Analyses using Human Primary Hepatocytes (January 21, 2014)
- Automated Fluorescence Imaging of GPCR Dependent Second-Messenger Systems (January 21, 2014)
- Validation of a 3-Dimensional Human Liver Microtissue Model for Long-term Hepatotoxicity Studies (January 21, 2014)
- Automated Tissue Culture Cell Fixation and Staining in Microplates (January 21, 2014)
- Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes (January 21, 2014)
- Investigation of Cell Migration using a High Density Cell Exclusion Assay and Automated Microplate Imager (January 21, 2014)
- Multiplexed Assay for IL-6 Secretion and Cell Viability using an Epithelial Ovarian Cancer Cell Line (January 21, 2014)
- Multiplexed Assay for IL-6 Secretion and Cell Viability using an Epithelial Ovarian Cancer Cell Line (May 3, 2013)
技术注释
- Agilent Seahorse XF Imaging & Cell Counting Software (June 28, 2018)
- Determination of Cell Confluence using Automated Cell Imaging for Verification of Cell Retention during Rigorous Cell Washing (December 20, 2017)
- Automated Cell Counting with High Contrast Brightfield and EVE Counting Slides (November 29, 2017)
- Top 10 Tips for Cellular Microscopy (June 8, 2015)
- Angled Dispenser Tips for Reagent Addition to Live Cells (June 16, 2014)
动态摘要
- Measuring Extracellular Oxygen Consumption Using a Soluble Fluorescent Biosensor (May 20, 2020)
- Characterizing Yeast Vitality (April 17, 2020)
- Analysis of RelA Nuclear Translocation (January 9, 2020)
- Multi-Mode Metabolic Profiling of Live Yeast Cells (November 14, 2019)
- Automated Image Analysis of Biovolume Using Robotics (October 7, 2019)
- Normalization of Agilent Seahorse XF Data (September 3, 2019)
- Automated Analysis of Neutrophil Netosis (April 25, 2019)
- 3D Kinetic NK Cell Cytotoxicity Assay (November 13, 2018)
- Automatic Transfection Efficiency Calculation in Microplates (November 13, 2018)
- Comet Assay: Single Cell Genotoxicity Measurement (November 13, 2018)
- Automated Wound Creation for Scratch Wound Healing Assays (November 13, 2018)
- Automated Cell Fixing, Staining and Immunofluorescence in Microplates (August 3, 2018)
- Cell Cycle Analysis Using a Nuclear Stain (July 31, 2018)
- Label-Free Tumor Invasion Assay Using 3D Tumor Spheroids (June 19, 2018)
- High Resolution Imaging of Cells Undergoing Mitosis in Microplates (April 6, 2018)
- High Throughput Cell Migration Assay (April 5, 2018)
- Direct Fluorescence Assay (DFA) (April 2, 2018)
- Measuring Apoptosis in Zebrafish Treated with Ethanol (March 26, 2018)
- Quantifying Cancer Biomarker Gene Expression Using RNA FISH (March 21, 2018)
- Live Cell Imaging of Apoptosis and Necrosis (March 20, 2018)
- Acid Fast Bacteria (AFB) Scoring (March 19, 2018)
- γH2AX Foci Spot Counting as a Determination of Genotoxicity (February 16, 2018)
- Phagocytosis Assay (February 16, 2018)
- Imaging and Quantification of T Cell Mediated Cytotoxicity (December 7, 2017)
- Image-Based Tracking and Quantification of T Cell Activation (December 7, 2017)
- Cell Proliferation - Label-free Confluence (November 15, 2017)
- Non-Alcoholic Steatohepatitis (NASH) Imaging Assay (November 6, 2017)
- Mitochondrial Membrane Potential Analysis Using Object Spot Counting (August 4, 2017)
- Kinetic Cytotoxicity Using Cell Counting (June 16, 2017)
- Autophagy Analysis Using Object Spot Counting (June 14, 2017)
- Cell Migration Assay: 3D Bioprinting (May 4, 2017)
- Cell Proliferation - Label-free Cell Counting (April 27, 2017)
- Fluorescence-based Plaque Assay (February 24, 2017)
网络视频
- The Multi-Faceted Assays Enabled with Cytation Technology - On Demand (September 17, 2020)
- 3D Culture Methods and Imaging Considerations - On Demand (April 29, 2020)
- Image Analysis 101 - Case Studies in Microbiology - On Demand (November 7, 2018)
- Combining Automated Liquid Handling, Cellular Microscopy and Magnetic Bioprinting to Create Walk Away 3D Oncology, Stem Cell Differentiation, and Toxicity Procedures (May 24, 2017)
- Phenotypic and MoA Analysis of Anti-Metastatic Molecules using 3D Spheroid-Based Tumor Invasion Assays, Kinetic Microplate Detection, and Cellular Microscopy (May 11, 2015)
- Phenotypic and MoA Analysis of Anti-Metastatic Molecules using 3D Tumoroid Invasion Assays, Kinetic Ligand Binding, and Cellular Microscopy (March 11, 2015)
- Food Quality Assurance: A Novel Seafood Freshness Assay (July 11, 2014)
- Comparison of 3D Cell Culture Technologies to Plated Cells in 2D for Oncology and Toxicology Applications (March 3, 2014)
- Cellular Imaging Made Easy (October 23, 2013)
- A New Look at Cell-based Assays in a Multi-Mode Microplate Reader using Fluorescence Microscopy (July 1, 2013)
白皮书
- Fluorescent Proteins - Filters, Mirrors and Wavelengths (July 7, 2017)
- 3D Cell Culture: A Review of Current Techniques (November 12, 2015)
- Sample Preparation for Fluorescence Microscopy: An Introduction - Concepts and Tips for Better Fixed Sample Imaging Results (July 28, 2015)
- An Introduction to Reactive Oxygen Species - Measurement of ROS in Cells (January 26, 2014)