Related App Note: Validation and Comparison of Single-Step Flash and Dual-Spectral Luciferase Reporter Gene Assays using the Synergy™ Line of Microplate Readers
Gen5 version required: 2.4 or higher
The Gen5 protocol used to control the reader and injection of luciferase assay regent as well as collection of luminescence data from the dual luciferase assay. The normalization reporter (red firefly luciferase) and experimental reporter (Gaussia luciferase) data are then plotted and subject to regression analysis. Data was also exported to for further analysis in both Excel (Microsoft, Redmond, CA) and Prism GraphPad (La Jolla, CA).