Variable Bandwidth Monochromators Technology

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  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
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  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
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  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Endotoxin Analysis
  Bacterial endotoxins are lipopolysaccharides (LPS) generally found in the outer membrane of gram-negative bacteria, such as E.coli, and are widely present in our environment. Because of the pyrogenic nature of endotoxins, with human effects from fever to hemorrhagic stroke, FDA regulations require that raw materials (e.g. water) used in parenteral drugs be tested for endotoxin levels.
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  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation™ and Lionheart™ FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5™ image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
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  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
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  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
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  Synergy Neo2 and Synergy H1 Now Available with Expanded Temperature Control Range
  July 28, 2021 - Now with temperature control up to 70 ºC, BioTek’s Synergy Neo2 Hybrid Multi-Mode Reader and Synergy H1 Hybrid Multi-Mode Reader aid in more assay workflows than ever before. Application flexibility extends to enzyme kinetics, protein stability and aggregation, and projects incorporating loop mediated isothermal amplification (LAMP) assays. 浏览更多
  
  BioTek's Cytation 7 Cell Imaging Multi-Mode Reader Named ‘Best New Drug Discovery & Development Product’ at Scientists’ Choice Awards
  May 5, 2021 - BioTek Instruments is pleased to announce that the Cytation 7 Cell Imaging Multi-Mode Reader was awarded ‘Best New Drug Discovery & Development Product’ of 2020. The honor was voted upon by SelectScience members across the world and celebrated at the 2021 Scientists’ Choice Awards online awards ceremony. 浏览更多
  
  BioTek Announces New Cytation C10 Confocal Imaging Reader
  February 2, 2021 - BioTek Instruments, a part of Agilent, is excited to unveil the Cytation C10, an advanced, automated Confocal Imaging Reader. This versatile and multi-functional automated system is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and quantitative data to support cell-based research. 浏览更多
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  Annual Meeting of the Platform for Stem Cell Research in Regenerative Medicine
  
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主页产品技术 Imager & Microplate Reader Technologies Variable Bandwidth Monochromators

Many multi-mode microplate readers use monochromator-based optics for fluorescence intensity measurements, allowing great flexibility to use multiple fluorescent dyes and stains. Most of these systems offer a fixed bandwidth, which works well for standard fluorophores but aren’t always optimal for some others, including those with a narrow Stokes’ Shift.

Synergy™ Neo2 Multi-Mode Reader, Synergy H1 Hybrid Multi-Mode Reader and Cytation™ 5 and Cytation 7 Cell Imaging Multi-Mode Readers have monochromator optics that incorporate user-selectable variable bandwidths for the best assay specificity and assay sensitivity. Synergy Neo2’s monochromators have a range from 3 nm to 50 nm, while the Synergy H1, Cytation 5 and Cytation 7 have a range of 9 nm to 50 nm; both ranges are selectable in 1 nm increments.

Click on the videos below to learn more about the benefits of variable bandwidth monochromators for fluorescence detection.

Synergy Neo2

Cytation 5 & 7

Synergy H1

Learn more about optimizing applications using variable bandwidth in BioTek’s multi-mode microplate readers.

Utility of Variable Bandwidth Monochromators for Quantification of Fluorescent Probes in Produced Effluent Water

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全自动细胞成像系统

Confocal Imaging Reader

细胞成像多功能检测系统

Multi-Plate Live Cell Imaging System

Peripherals

产品
比较表

全功能微孔板检测仪

Multi-Plate Absorbance Reader

吸收光检测仪

Peripherals

产品比较表

多功能洗板分液器

微孔板洗板机

微孔板分液器

液体处理系统

产品比较表

自动化系统

产品比较表

操作软件

Software Apps

产品比较表

Multi-Mode Detection Technologies

Imager & Microplate Reader Technologies

Liquid Handling Technologies

Synergy Neo2 and Synergy H1 Now Available with Expanded Temperature Control Range

BioTek's Cytation 7 Cell Imaging Multi-Mode Reader Named ‘Best New Drug Discovery & Development Product’ at Scientists’ Choice Awards

BioTek Announces New Cytation C10 Confocal Imaging Reader

Neuroscience (SfN) 2021

November 8 - 16, 2021

Booth #1635

McCormick Place Convention Center

SITC 2021

November 10 - 14, 2021

Walter E. Washington Convention Center

Annual Meeting of the Platform for Stem Cell Research in Regenerative Medicine

November 12, 2021

Variable Bandwidth Monochromators

This Technology is Available in: