product design

Cytation 7 Cell Imaging Multi-Mode Reader

技术细节

General

Detection modes UV-Vis absorbance
Fluorescence intensity
Luminescence
Read methods Endpoint, kinetic, spectral scanning, well area scanning
Microplate types Monochromator: 6- to 384-well plates
Imaging: 6- to 1536-well plates
Other labware supported Microscope slides, Petri and cell culture dishes, cell culture flasks (T25), counting chambers (hemocytometer)
Take3 Micro-Volume Plates
Temperature control 4-Zone incubation to 45 °C with Condensation Control; +0.5 °C at 37 °C
Cooling Optional Peltier Cooling Module maintains internal temperature with < 1 °C rise over ambient. Provides internal cooling after incubated processes.
Shaking Linear, orbital, double orbital
Software Gen5™ Microplate Reader and Imager Software included
Gen5 Secure for 21 CFR Part 11 compliance (option)
Automation BioStack and 3rd party automation compatible
BioSpa 8 Automated Incubator compatible
CO2 and O2 control (option) Range: 0 - 20% (CO2); 1 - 19% (O2)
Models for both CO2 and O2 or CO2 only are available

Imaging - Inverted Microscope

Imaging mode Fluorescence, color brightfield, user-selectable brightfield/high contrast brightfield
Imaging method Single color, multi-color, time lapse, montage, z-stacking, z-stack montage
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS wide field of view monochrome camera
Objective capacity 6-position automated turret for user-replaceable objectives
Objectives available 1.25x, 2.5x (2.25x eff),2.5x (2.75x eff), 4x,10x, 20x, 40x, 60x
Image filter cube capacity 4 user-replaceable fluorescence cubes, plus brightfield channel
Imaging filter cubes available DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-FRET, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, Tag BFP-FRET, GFP (Ex)-CY5 (Em), RFP (Ex)-CY5 (Em), Alexa 568, Ex 377/Em 647, Oxidized roGFP2, TRITC
Imaging LED cubes available 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 554 nm, 590 nm, 623 nm, 655 nm, 740 nm
Automated functions Autofocus, user-trained autofocus, autoexposure, auto-LED intensity
Autofocus method Image-based autofocus
User-trained autofocus
Laser autofocus (option)
Positional controls Software Control
Joystick controller (option)
Image collection rate Image-based autofocus:
96 wells, 1 color (DAPI), 4x, 6 minutes

Laser autofocus:
96 wells, 1 color (DAPI), 4x, <3 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features

Imaging - Upright Microscope

Imaging modes Reflected color brightfield; transmitted color brightfield
Imaging methods Single image, montage, time lapse, Z-stacking
Image processing Z-projection, digital phase contrast, stitching
Camera 16-bit Sony CMOS wide field of view color camera
Lenses Finder scope, 2x, 4x, 8x
Positional controls Software Control
Joystick controller (option)
Image collection rate Entire 100 mm dish (montage) @ 1x: <3:00 minutes
Entire microscope slide (montage) @ 1x: <1:15 minutes
96-well ELISpot plate @ 1x: <5 minutes
Image Analysis Software option Gen5 Image+: Image analysis
Gen5 Image Prime: Advanced image analysis
Gen5 Secure: 21 CFR Part 11 compliant features

Fluorescence Intensity

Light source Xenon flash
Detector PMT (red-shifted PMT option)
Wavelength selection Quad monochromators (top/bottom)
Wavelength range 250 – 700 (900 nm option)
Monochromator bandwidth Variable, from 9 nm to 50 nm in 1 nm increments
Dynamic range 7 decades
Sensitivity Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) – bottom
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Luminescence

Wavelength range 300 - 700 nm
Dynamic range >6 decades
Sensitivity 20 amol ATP (flash)

Absorbance

Light source Xenon flash
Detector Photodiode
Wavelength selection Monochromator
Wavelength range 230 - 999 nm, 1 nm increment
Monochromator bandwidth 4 nm (230 - 285 nm), 8 nm (>285 nm)
Dynamic range 0 - 4.0 OD
Resolution 0.0001 OD
Pathlength correction Yes
Monochromator wavelength accuracy +2 nm
Monochromator wavelength repeatability +0.2 nm
OD accuracy <1% at 3.0 OD
OD linearity <1% from 0 to 3.0 OD
OD repeatability <0.5% at 2.0 OD
Stray light 0.03% at 230 nm
Reading speed (kinetic) 96 wells: 11 seconds
384 wells: 22 seconds

Reagent Injectors (option)

Number 2 syringe pumps
Supported labware 6- to 384-well plates, Petri and cell culture dishes
Dead volume 1.1 mL with back flush
Dispense volume 5 - 1000 µL in 1 µL increments
Plate geometry 6- to 384-well microplates
Dispense accuracy +1 µL or 2%
Dispense precision <2% at 50 - 200 µL

Physical Characteristics

Power 150 Watts maximum consumption
Dimensions 20.2" D x 16.4" W x 17.5" H
(51.4 cm D x 41.6 cm W x 44.5 cm H)
Weight 80 lbs (36.3 kg)

Regulatory

Regulatory CE and TUV marked. RoHS Compliant. Models for In Vitro Diagnostic use are available.
link