ELISA and Related Immunoassays

ELISA (Enzyme-Linked-Immunosorbent-Assay) is a very common, robust technique for detecting various analytes. The ELISA technique causes formation of specific immune complexes that can be measured. There are wash and no-wash immunoassays using traditional enzyme-linked technologies with colorimetric, fluorometric or luminometric detection, TR-FRET or HTRF, AlphaScreen and AlphaLISA. ELISAs are typically run in 96- to 1536-well microplates, but can also be performed in micro-volumes using specialized very low volume plates like the Take3 Micro-Volume Plate. The basic “building blocks” of an ELISA reaction are:

BioTek has developed several application notes describing a variety of ELISA applications.

Application Notes:

• Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis
• Concentration and Detection of Low Levels of Escherichia coli O157:H7, Listeria monocytogenes 4b, and Salmonella enterica Typhimurium in High Organic Load Lettuce Wash
• Using EL406™ Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation