Resources
194 结果:
应用手册
3D Cell Culture (23)
In-vitro cell-based assays that utilize a 3D structure of aggregated cells using hydrogels, scaffolds or spheroids to provide more physiologically relevant data.
- Combination of a Fluorescent Substrate-Based MMP Activity Assay and Hit Pick Reading/Imaging Procedure (March 25, 2022)
- Automated Image Screening of Zebrafish Embryos Exposed to Developmental Toxins (January 4, 2019)
- Automated Media Exchange for Spheroid Cultures Using a Novel MultiFlo FX Accessory (July 31, 2018)
- 3D Spheroid-Based Tumor Invasion Assay (July 16, 2018)
- Brightfield and Fluorescence Imaging using 3D PrimeSurface® Ultra-Low Attachment Microplates (December 21, 2017)
- An Image-Based Method to Detect and Quantify T Cell Mediated Cytotoxicity of 2D and 3D Target Cell Models (December 13, 2017)
- Cost-Effective Automated Hepatotoxicity Testing using in vitro 3D Bioprinting (August 17, 2017)
- Automated Label-Free Cell Migration Assays using Magnetic 3D Bioprinting (August 16, 2017)
- Long-Term Hepatotoxicity Studies using Cultured Human iPSC-Derived Hepatocytes (August 15, 2017)
- Validation of an Image-Based 3D Natural Killer Cell Mediated Cytotoxicity Assay (March 24, 2017)
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids (December 16, 2016)
- Automation and Image-Based Analysis of a Hanging Drop Microhole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation (October 25, 2016)
- Automated Monitoring of Protein Expression and Metastatic Cell Migration using 3D Bioprinted Colorectal Cancer Cells (September 23, 2016)
- Using Automated Imaging and Advanced 3D Cell Culture Techniques to Quantify Apoptosis Activity (April 5, 2016)
- Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential Neurotoxicants (March 25, 2016)
- Validation of High-Throughput Wound Healing Assay using 3D Cell Patterning and Automated, Kinetic Imaging (February 8, 2016)
- Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses - Comparison of Results Generated with an in vitro Cell Signaling Assay using Cells Cultured in 2D and a Novel 3D Cell Culture System (April 30, 2015)
- Automation of HTRF elF4E Kinase Assay Using a 3D Tumoroid- Based Cell Model (April 30, 2015)
- Combining Kinetic Ligand Binding and 3D Tumor Invasion Technologies to Assess Drug Residence Time and Anti-metastatic Effects of CXCR4 Inhibitors (April 16, 2015)
- Quantification of MMP Activity and Inhibition in a 3D Tumor Invasion Model (April 7, 2015)
- Z-Stacking of Single Plane Digital Widefield Fluorescent Images (October 31, 2014)
- Live Cell Hypoxia Determination in a 3D Spheroid Model (August 5, 2014)
- The Effect of Cell Culture Method on Long-Term Primary Hepatocyte Cell Health (February 19, 2014)
ADME/Tox (16)
In-vitro assays using either cells or purified enzyme for Lead Optimization of small molecules in drug discovery; drug absorption, drug metabolism and drug cytotoxicity assays
- Kinetic Degradation of IMiD Molecular Glues and Their Target Families (March 17, 2022)
- Cell Viability Assessment by LUCS Assay (March 17, 2022)
- Automated Image Screening of Zebrafish Embryos Exposed to Developmental Toxins (January 4, 2019)
- Comparison of Oridonin Cytotoxicity in U-2 OS and HepG2 Cells (July 6, 2016)
- Oridonin Perfusion Causes Cytotoxicity in U-2 OS Cells (June 29, 2016)
- Detection of Human IgG Using a Luminescence-Based Proximity Assay (December 5, 2014)
- The Impact of a 3-Dimensional Human Liver Microtissue Model on Long-term Hepatotoxicity Studies (March 31, 2014)
- Incorporation of a Novel 3D Cell Culture System to Perform in vitro Cytotoxicity Analyses using Human Primary Hepatocytes (March 20, 2014)
- Analysis of a New High Throughput Screening Detection Technology for Rapid hERG Safety Testing using a Fluorescence Polarization Assay (February 12, 2013)
- Overcoming the Crabtree Effect is Critical to Mitochondrial Toxicity Testing (October 24, 2011)
- Low Cost Solution for PAMPA Assays - Using the Epoch Microplate Spectrophotometer with pION's PAMPA Explorer™ for the In-vitro Drug Permeability Studies (June 16, 2011)
- A Fluorescent Microplate-Based Assay Workflow Enabling the Functional Characterization of Multi-Drug Resistance Transporters in Living Cells - Characterization of MDR Antiporter Expression in Tissue Culture Cell Lines (June 3, 2011)
- Automated Detection of Drug-Induced Lysosomal Cytotoxicity (May 21, 2010)
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Automated Drug-Induced Chromatin Condensation Detection
Detection of End-Stage Apoptosis Using the Nuclear-ID Kit from Enzo Life Sciences (February 17, 2010) - Quantifying Cytotoxicity of Thiostrepton on Mesothelioma Cells using MTT Assay and the Epoch Microplate Spectrophotometer (December 10, 2009)
- An Absorbance-based Cytotoxicity Assay using High Absorptivity, Water-soluble Tetrazolium Salts - Cell Quantitation Using WST-8 and the Synergy™ Mx (March 20, 2009)
Automation (23)
Demonstration of automated procedures for various assays using microplates.
- Kinetic Degradation of IMiD Molecular Glues and Their Target Families (March 17, 2022)
- Characterization of LAMP Assays Using a Multimode Microplate Reader (September 15, 2021)
- Improved LAMP Assay Performance with Increased Temperature (September 15, 2021)
- Automating the Wash Steps of ELISpot Assays using an Agilent BioTek 405 TS Microplate Washer (March 18, 2020)
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format (December 26, 2019)
- A High-Throughput Luminescence-Based Live Cell Assay to Measure ß-Arrestin Recruitment in Real Time (April 4, 2019)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- Automated Media Exchange for Spheroid Cultures Using a Novel MultiFlo FX Accessory (July 31, 2018)
- Cost-Effective Automated Hepatotoxicity Testing using in vitro 3D Bioprinting (August 17, 2017)
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids (December 16, 2016)
- Automation and Image-Based Analysis of a Hanging Drop Microhole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation (October 25, 2016)
- Applied Robotics for Enhanced Throughput Options in Microscopy (November 6, 2014)
- Automated Washing of Multiplex Bead Based Assays for the Luminex MAGPIX® Reader System - Comparison of Luminex 100 and MAGPIX Luminex Readers (July 26, 2011)
- Multiplexing Magnetic Bead-based Human Cytokine Assays - Automated Magnetic Bead Washing Using the ELx405 (July 13, 2010)
- Automated Cell Dispensing into 1536-Well Microplates for HTS - Using the MicroFlo™ Select to Seed Tissue Culture Cells (February 18, 2010)
- Versatile, Cost-Effective Automation of Avian Influenza and Mycoplasma Gallispeticum-Synovaie ELISAs (January 28, 2010)
- Automated Workflows for Luminex xMAP® Assays (November 6, 2009)
- Automated Magnetic Bead Washing of Metabolic Hormone Assays - Using the ELx405 Magnetic Bead Washer to Wash MILLIPLEX MAP Assays (September 22, 2009)
- Automated Washing of Magnetic Bead QuantiGene Plex 2.0 Assays - Using the ELx405 Magnetic Bead Washer to Wash Multiplexed mRNA Quantitation Assays (September 18, 2009)
- Using the Agilent BioTek EL406 Combination Washer Dispenser to Semi-Automate a Competitive ELISA for Melamine (January 16, 2009)
- Aliquoting Agar-Containing Solutions with the Agilent BioTek MicroFlo Select Dispenser (April 28, 2008)
- Luminex xMAP Assays: Using BioTek’s ELx50 Filter Microplate Washer to Perform the Wash Processes Required (April 16, 2007)
Biochemical Assays (9)
Assays using purified drug target in Hit ID and Hit to Lead small molecule screening efforts.
- Characterization of LAMP Assays Using a Multimode Microplate Reader (September 15, 2021)
- Improved LAMP Assay Performance with Increased Temperature (September 15, 2021)
- Receptor-Mediated Stimulation of AKTpan-kinase in NIH3T3 Cells (October 8, 2020)
- Rapid Measurement of IgG Using Fluorescence Polarization (September 15, 2017)
- Monitoring of Protein Kinase Activity Using Next Generation CSox-based Substrate Sensors (August 2, 2017)
- Comparative Analysis of Dual-Luciferase Assay Technologies in a High Throughput Microplate Format (March 18, 2016)
- Analysis of α-Synuclein Fibril Formation in vitro (December 10, 2014)
- Detection of Human IgG Using a Luminescence-Based Proximity Assay (December 5, 2014)
- Mycoplasma Detection Using Synergy 2 Multi-Mode Microplate Reader and the Lonza MycoAlert Assay (December 8, 2006)
Biofuel Research (6)
Assays assisting in R&D efforts to produce biofuels from corn, lignocellulosic material and algae.
- Chemical and Biochemical Means to Detect Alcohol - Determination of Ethanol Concentration in Fermented Beer Samples and Distilled Products (May 29, 2012)
- Enzymatic Digestion of Polysaccharides (Part I) (February 27, 2012)
- Enzymatic Digestion of Polysaccharides (Part II) (February 27, 2012)
- Monitoring Enzymatic Glucose Production from Cellulosic Feedstock (December 19, 2011)
- Determination of Algal Cell Lipids Using Nile Red - Using Microplates to Monitor Neutral Lipids in Chlorella Vulgaris (July 12, 2011)
- Monitoring of Algal Growth Using Their Intrinsic Properties (July 11, 2011)
Biomarkers (9)
Quantification of biological markers (i.e. proteins, DNA, RNA, etc.) associated with disease, toxicity and drug efficacy from biofluids (i.e. serum/plasma).
- Expression and Intracellular Translocation of Cancer Biomarkers in Hepatocarcinoma Cells Induced by Changes in Mitochondrial Metabolism (November 8, 2022)
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- Stimulation of IL-2 Secretion in Human Lymphocytes (March 21, 2018)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (March 21, 2016)
- High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model (February 18, 2016)
- Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging (February 18, 2016)
- Differential Cancer Biomarker Secretion of Cultured Cells - Use of the ELx50 to Automate the Wash Steps of Multiplexed Luminex® xMAP® Bead Assays (July 26, 2011)
- AlphaLISA Quantitation of Human Insulin Using a Hybrid Agilent BioTek Synergy 4 Multimode Microplate Reader (December 10, 2007)
- Cholesterol Quantification Using a Fluorometric Assay and Synergy HT Multi-Mode Microplate Reader (February 28, 2005)
Biotherapeutics (8)
Microplate assays used for biologic drug discovery and development: clonal selection, characterization, formulation studies and immunogenicity.
- Comparison of Assay Methods for the Detection of Residual Protein A in Biological Therapeutics (July 14, 2015)
- Automation of a Homogeneous Proximity Assay for Detection of Residual Protein A in Biological Therapeutics (November 14, 2014)
- An Automated DELFIA ADCC Assay Method using a CD16.NK-92 Cell Line (March 7, 2013)
- Automated Bioluminescent ADCC Reporter Bioassay Using Bioengineered Jurkat Cells (February 8, 2013)
- A Semi-Automated, Non-Radioactive Assay for the Detection of Antibody-Based Complement-Dependent Cytotoxicity (March 26, 2012)
- Automated Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cell- Mediated Cytotoxicity Utility (February 3, 2012)
- Semi-Automation of a Nonradioactive Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Assay (January 31, 2012)
- A Novel Protein Aggregation Assay for Biologics Formulation Studies and Production QA/QC - ProteoStat® Fluorescent Reagent for Microplate-based Aggregate Quantitation (March 18, 2011)
Cell-based Assays (33)
Quantitative assays of cellular function using a wide range of microplate reader optical detection modes.
- Expression and Intracellular Translocation of Cancer Biomarkers in Hepatocarcinoma Cells Induced by Changes in Mitochondrial Metabolism (November 8, 2022)
- High-Throughput Imaging and Automated Analysis of Focal Adhesions (August 1, 2022)
- Quantitative Assessment of Antioxidant Activities in Living Cells (April 11, 2022)
- Combination of a Fluorescent Substrate-Based MMP Activity Assay and Hit Pick Reading/Imaging Procedure (March 25, 2022)
- Kinetic Degradation of IMiD Molecular Glues and Their Target Families (March 17, 2022)
- Cell Viability Assessment by LUCS Assay (March 17, 2022)
- Automating a Direct, Cell-Based, Target-Compound Interaction Assay for Methyltransferase and Bromodomain Proteins using the InCELL Platform (February 26, 2021)
- Receptor-Mediated Stimulation of AKTpan-kinase in NIH3T3 Cells (October 8, 2020)
- Automated Colony Formation Assay (May 1, 2020)
- A Homogeneous FRET-based HTS Assay for Quantification of pRb in Cancer Cell Lines to Monitor Inhibition of G0/ G1 Cell Phase Transition (April 21, 2020)
- Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress (March 25, 2020)
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- High-Throughput Fluorescent Colony Formation Assay (January 10, 2020)
- Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format (December 26, 2019)
- Automated Label-Free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay (May 21, 2019)
- A High-Throughput Luminescence-Based Live Cell Assay to Measure ß-Arrestin Recruitment in Real Time (April 4, 2019)
- Automated Image Screening of Zebrafish Embryos Exposed to Developmental Toxins (January 4, 2019)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- Investigation of Protein: Protein Interactions (PPI) using a Novel Bioluminescence Resonance Energy Transfer (BRET) Proximitybased, High Throughput Screening Assay (June 17, 2016)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (March 21, 2016)
- High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model (February 18, 2016)
- Automation of a Homogeneous Proximity Assay for Detection of ERK1/2 or SMAD3 Phosphorylation (July 14, 2015)
- Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging (April 13, 2015)
- Live Cell Imaging of GPCR Dependent Second-Messenger Systems (April 14, 2014)
- Validation and Comparison of Single-Step Flash and Dual-Spectral Luciferase Reporter Gene Assays using the Synergy™ Line of Microplate Readers (October 16, 2013)
- Multiplexed Assay for IL-6 Secretion and Cell Viability Using an Epithelial Ovarian Cancer Cell Line (April 15, 2013)
- Determination of Endogenous Cellular Kinase Activity Through the Use of an Automated, High Throughput AlphaScreen-Based Assay (September 13, 2012)
- Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis (May 16, 2012)
- HTRF Ligand Binding Assay for the Chemokine Receptor CXCR4 (October 29, 2010)
- Live-Cell Assay to Interrogate GPCRs by Monitoring cAMP Levels using a Bioluminescent Readout (October 19, 2010)
- Automated Processing of a Homogeneous Phospho-ERK1/2 Assay for Use as a Generic GPCR Sensor - Homogeneous LanthaScreen® Cellular Assay (February 1, 2010)
- HTRF® cAMP/IP-One HTplex™ Cell-Based Assay performed on BioTek’s Synergy™ 2 Multi-Mode Microplate Reader (June 17, 2009)
- Oris - A Novel High Throughput-Compatible Cell Migration Screening Assay (August 21, 2008)
Cellular Imaging (40)
Imaging of cells after fixation or lysis using fluorescence microscopy.
- Expression and Intracellular Translocation of Cancer Biomarkers in Hepatocarcinoma Cells Induced by Changes in Mitochondrial Metabolism (November 8, 2022)
- High-Throughput Imaging and Automated Analysis of Focal Adhesions (August 1, 2022)
- Combination of a Fluorescent Substrate-Based MMP Activity Assay and Hit Pick Reading/Imaging Procedure (March 25, 2022)
- Whole-Mount Immunofluorescence Imaging of Zebrafish Eye Structures (September 21, 2021)
- Whole-Organism Imaging of Spinal Motor Neurons and Musculature with the Cytation C10 Confocal Imaging Reader (May 27, 2021)
- Image Analysis of Herpesvirales Specificity Challenge using Direct Fluorescence Antibody Assay (DFA) (December 10, 2020)
- Detection and Automated Imaging of Regions of Interest (ROIs) when Performing Whole Slide Imaging (WSI) (May 15, 2020)
- Automated Colony Formation Assay (May 1, 2020)
- Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress (March 25, 2020)
- Stimulation of Human Peripheral Blood Mononuclear Cells (March 10, 2020)
- High-Throughput Fluorescent Colony Formation Assay (January 10, 2020)
- Automated Scoring of Acid-fast Bacteria (AFB) by Fluorescent Smear Microscopy (October 16, 2019)
- Nuclear Translocation of RelA in Stimulated Macrophages (September 18, 2019)
- Quantitative Image Analysis of Bacilli Viability in Agar Mounted Soil Samples (July 10, 2019)
- Automated Label-Free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay (May 21, 2019)
- Automated Analysis of Neutrophil NETosis Activity (April 30, 2019)
- Analyzing Wound Healing in Zebrafish Embryos (February 11, 2019)
- Quantitative Microscopy of Angiogenesis in Zebrafish Embryos (December 6, 2018)
- Determination of Cell Cycle Time (December 6, 2018)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System (September 5, 2018)
- Monitoring Cell Cycle Progression in Cancer Cells (July 3, 2018)
- Stimulation of IL-2 Secretion in Human Lymphocytes (March 21, 2018)
- Using Acridine Orange to Measure Cell Death in Ethanol Treated Zebrafish Embryos (December 27, 2017)
- Automated Imaging and Dual-Mask Spot Counting of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis (December 19, 2017)
- Monitoring Cell Cycle Progression Using Microscopy (August 22, 2017)
- Lipid Accumulation in HepG2 Cells Exposed to Free Fatty Acids (August 14, 2017)
- Comparison of Different Cell Types for Neutral Lipid Accumulation (August 14, 2017)
- Automation of Non-Alcoholic Fatty Liver Disease Model Assay (August 14, 2017)
- Automated Monitoring of Protein Expression and Metastatic Cell Migration using 3D Bioprinted Colorectal Cancer Cells (September 23, 2016)
- Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis (July 7, 2016)
- Analysis of the Effect of Aggregated β-Amyloid on Cellular Signaling Pathways Critical for Memory in Alzheimer’s Disease (March 21, 2016)
- Detection and Visual Localization of Individual Cellular Proteins Using a Proximity Ligation Assay (March 4, 2016)
- Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging (February 18, 2016)
- Analysis of Cell Retention Following Rigorous Automated Cell Fixation and Staining (December 18, 2015)
- Automated Imaging and Analysis of a Novel Comet Assay to Enable High Throughput Genotoxicity Testing (December 1, 2015)
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy (April 10, 2015)
- High Resolution Fluorescence Microscopy of PtK2 Cells Undergoing Mitosis in Microplates (June 24, 2014)
- Analysis of Nuclear Stained Cells - Using the Cytation™3 Cell Imaging Multi-Mode Microplate Reader with DAPI-Stained Cells (April 15, 2013)
- Automated Tissue Culture Cell Fixation and Staining in Microplates (April 15, 2013)
Enzymology (5)
Kinetic studies using purified enzymes in a microplate format.
- Monitoring Protein Dehydrogenase Activity with Filter-based Absorbance Spectroscopy (September 22, 2017)
- Monitoring of Protein Kinase Activity Using Next Generation CSox-based Substrate Sensors (August 2, 2017)
- Determining NADH Concentrations with Synergy 2 Multi-Mode Microplate Reader using Fluorescence or Absorbance (November 26, 2007)
- Kinetic Analysis of ß-Galactosidase Activity using PowerWave HT Microplate Spectrophotometer and Gen5™ Data Analysis Software (February 26, 2007)
- Caspase Activity Using Synergy HT Multi-Mode Microplate Reader: Measuring Apoptosis Enzymes (July 28, 2004)
Epigenetics (3)
Cell-based and biochemical assays to measure activity and small molecule modulation of epigenetic target proteins.
- Automating a Direct, Cell-Based, Target-Compound Interaction Assay for Methyltransferase and Bromodomain Proteins using the InCELL Platform (February 26, 2021)
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy (April 10, 2015)
- Automated, High Throughput, HTRF-Based Detection of Histone Methyltransferase and Demethylase Enzyme Activity (March 5, 2013)
Food Safety & Quality (9)
Assays measuring contaminants in food and beverages including bacteria and processing by-products that influence customer health and product quality.
- Quantitative Assessment of Antioxidant Activities in Living Cells (April 11, 2022)
- Characterization of LAMP Assays Using a Multimode Microplate Reader (September 15, 2021)
-
A Novel Food Freshness Assay Based on ATP Depletion
Part 2: Challenge Testing of the Automated Assay (August 13, 2014) -
A Novel Food Freshness Assay Based on ATP Depletion
Part 1: Automation and Sample Prep Optimization (August 8, 2014) - Concentration and Detection of Low Levels of Escherichia coli O157:H7, Listeria monocytogenes 4b, and Salmonella enterica Typhimurium in High Organic Load Lettuce Wash (March 6, 2014)
- Determination of Antioxidant potential using an Oxygen Radical Absorbance Capacity (ORAC) Assay with Synergy H4 (August 7, 2012)
- Chemical and Biochemical Means to Detect Alcohol - Determination of Ethanol Concentration in Fermented Beer Samples and Distilled Products (May 29, 2012)
- Using the Agilent BioTek EL406 Combination Washer Dispenser to Semi-Automate a Competitive ELISA for Melamine (January 16, 2009)
- Analysis of Histamine in Wine Samples Using the Microplate Format (August 29, 2006)
Histology/Slide Scanning (3)
The study of the microscopic anatomy of tissues by sectioning and staining, typically with colored dyes such as H&E using an optical microscope.
Live Cell Imaging (38)
Kinetic imaging of live cells under environment control using brightfield, high contrast brightfield. phase contrast and fluorescence microscopies.
- Combination of a Fluorescent Substrate-Based MMP Activity Assay and Hit Pick Reading/Imaging Procedure (March 25, 2022)
- Monitoring pH in Long-term Proliferation Assays (August 20, 2020)
- Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress (March 25, 2020)
- Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format (December 26, 2019)
- Nuclear Translocation of RelA in Stimulated Macrophages (September 18, 2019)
- Automated Label-Free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay (May 21, 2019)
- Automated Analysis of Neutrophil NETosis Activity (April 30, 2019)
- α-SMA Expression in Hepatic Stellate Cells (December 21, 2018)
- Quantitative Microscopy of Angiogenesis in Zebrafish Embryos (December 6, 2018)
- Advanced Wound Healing Assay Workflow using Automated Scratch Wound Creation, High Contrast Brightfield and Fluorescence Kinetic Imaging (December 6, 2018)
- Thapsigargin-induced Cellular Stress Response and Inhibition of Gq-dependent Calcium Signaling (October 5, 2018)
- Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays (October 1, 2018)
- 3D Spheroid-Based Tumor Invasion Assay (July 16, 2018)
- Characterizing Gs- and Gi-dependent Regulation of cAMP Levels in Real Time with cADDis Biosensor and Automated Live-Cell Imaging (March 29, 2018)
- Live Cell Imaging of Apoptosis and Necrosis (March 23, 2018)
- Using Phenol Red to Assess pH in Tissue Culture Media (March 22, 2018)
- Automated, Multiparameter, Kinetic Methods to Quantify Cell Death (March 22, 2018)
- Image-Based, Label-Free Methods to Detect and Quantify General and Directed T Cell Activation (December 8, 2017)
- Automated Imaging Assay for Characterizing Ca2+ Flux with R-GECO Biosensor (September 28, 2017)
- Mitochondrial Membrane Potential by Object Spot Counting (September 11, 2017)
- Monitoring Cell Cycle Progression Using Microscopy (August 22, 2017)
- Autophagy Analysis Using Object Spot Counting (July 11, 2017)
- Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5 (May 16, 2017)
- Characterizing Calcium Mobilization using Kinetic Live Cell Imaging (January 26, 2017)
- Kinetic Proliferation Assay Using Label-Free Cell Counting (January 25, 2017)
- Monitoring Saccharomyces Cerevisiae Growth with Brightfield Microscopy in Real Time (July 20, 2016)
- Monitoring Viral Infection of Mammalian Cells using Digital Fluorescence Microscopy (July 5, 2016)
- Performance of a Label-Free Image-Based 2D Scratch Wound Healing Assay to monitor Cell Migration and its Inhibition (June 29, 2016)
- Monitoring Cytotoxicity by Oridonin in U-2 OS Cells in Real Time (May 20, 2016)
- Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging (April 13, 2015)
- Imaging and Analysis of a High Density Cell Migration Assay (November 25, 2014)
- Automated Hemocytometer‑Based Live/Dead Cell Counting Using Phase Contrast and Color Brightfield Imaging (November 4, 2014)
- Use of Phase Contrast Imaging to Track Morphological Cellular Changes due to Apoptotic Activity (October 31, 2014)
- Cell Synchrony Determination Using Microscopic Imaging - Use of Nuclear Staining to Assess Cell Cycle Stage by Nuclear DNA Content (October 14, 2014)
- Automated Kinetic Imaging of Cell Migration and Invasion Assays using Corning FluoroBlok Permeable Supports (October 9, 2014)
- Live Cell Imaging of GPCR Dependent Second-Messenger Systems (April 14, 2014)
- Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes (June 13, 2013)
- Imaging of BacMam Transfected U-2 OS Cells (April 15, 2013)
Nucleic Acid Quantification (12)
Quantification of dsDNA, ssDNA, RNA and µRNA using micro-volume, microplate and 1 cm pathlength devices for downstream processes such as PCR, qPCR and sequencing.
- Quantification of dsDNA using Qubit® dsDNA Broad Range (BR) Assay Kit (December 6, 2018)
- Fluorometric Quantitation of dsDNA using PicoGreen (January 25, 2017)
- Low-Volume, High-Throughput Workflow for Analysis of Nucleic Acid Samples for Biobanking (December 18, 2015)
- Microvolume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning (June 5, 2012)
- Analytical Performance of a Higher Throughput Micro-Volume Microplate Accessory for Microplate Spectrophotometer Platforms (November 10, 2011)
- Micro-Volume PicoGreen DNA Quantification using BioTek’s Take3 Multi-Volume Plate (June 2, 2010)
- Analytical Performance of Nucleic Acid Microvolume Quantification Using the Epoch Spectrophotometer System (December 9, 2009)
- Compatibility of Synergy™ Multi-Mode Microplate Readers for Micro-Volume Nucleic Acid Quantification (November 9, 2009)
- Multi-Volume Analysis of Nucleic Acids Using the Epoch™ Spectrophotometer System (November 3, 2009)
- Low Volume Nucleic Acid Quantification using a Multi-Volume Spectrophotometer System (August 17, 2009)
- BioTek PowerWave HT Microplate Spectrophotometer: Quantitation of Nucleic Acids (February 6, 2001)
- Nucleic Acid Purity Assessment Using A260/A280 Ratios (February 6, 2001)
Physical Sciences (2)
Determination of fundamental constants of physical chemistry.
Protein Quantification (11)
Quantification of total protein using micro-volume, microplate and 1 cm pathlength devices for downstream processes such as PAGE and mass spectrometry.
- Receptor-Mediated Stimulation of AKTpan-kinase in NIH3T3 Cells (October 8, 2020)
- Rapid Measurement of IgG Using Fluorescence Polarization (September 15, 2017)
- Protein Quantitation using NanoOrange Fluorescence (January 26, 2017)
- Automation of a Homogeneous Proximity Assay for Detection of Residual Protein A in Biological Therapeutics (November 14, 2014)
- UV Fluorescence Polarization as a Means to Investigate Protein Conformational and Mass Change - Using Intrinsic Tryptophan Fluorescence in Conjunction with UV-capable Polarizers (October 13, 2014)
- Micro-Volume Determination of Labeling Efficiency Using Spectral Scanning Analysis in a Standard Microplate Reader (February 11, 2013)
- Microvolume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning (June 5, 2012)
- Analytical Performance of a Higher Throughput Micro-Volume Microplate Accessory for Microplate Spectrophotometer Platforms (November 10, 2011)
- In-situ Micro-Volume Bicinchoninic Acid Protein Assay (February 26, 2010)
- Analytical Performance of Epoch™ Multi-Volume Spectrophotometer System for Protein Quantification (January 21, 2010)
- Peptide and Amino Acid Quantification Using UV Fluorescence in Synergy HT Multi-Mode Microplate Reader (April 18, 2003)
Stem Cell Differentiation (2)
Assay workflows that monitor and validate stem cell differentiation.
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids (December 16, 2016)
- Automation and Image-Based Analysis of a Hanging Drop Microhole Plate Model to Create and Differentiate 3D Mesenchymal Stem Cell Spheroids for Downstream Tissue Formation (October 25, 2016)
Suspension Cell Growth (3)
Spectrophotometric measurements of bacteria, yeast and other non-adherent cells based on light scatter, typically at A600.
- Monitoring Bacterial Growth under Different Environmental Conditions (July 16, 2020)
- Monitoring Cell Growth in 2 μL Volumes Using a Microplate Reader (December 19, 2011)
- Monitoring Growth of Beer Brewing Strains of Saccharomyces Cerevisiae - The Utility of Synergy H1 for Providing High Quality Kinetic Data for Yeast Growth Applications (November 17, 2010)
Veterinary Analysis (1)
Assays used in animal husbandry to assess health and disease.