Gen5 version required: 2.0 or higher
Gen5 was used to determine the AlphaLISA signal for analysis of the extent to which circulating drug interferes with assay performance due to competition between circulating drug and the assay antigen for ADAs. The sample file contains the read parameters used for this assay. The protocol contains an absorbance read step in an unused well to allow for a delay prior to reading the Alpha signal. Data was collected for statistical analysis after export to Microsoft Excel.