ELISA - BioTek Applications

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Peripherals
- Peripherals
  BioTek offers many peripheral instruments and accessories to expand functionality and enhance assay workflows. Many peripheral instruments and modules are compatible with several different instruments, see the list here.
- 配件
  To complete or enhance your BioTek imager, microplate reader, liquid handling or robotics system, we offer a wide range of accessories, from tubing and bottles to imaging filter cubes. Search our Accessory database for Accessories compatible with your instrument. Search by Keyword, Part #, Product or Accessory Type.
- Application Starter Kits
  BioTek’s Application Starter kits contain everything you’ll need to implement the latest assays for a particular application, quickly and with minimal training. Kits contain hardware, software, consumables and reagents all in a convenient kit.
应用
- Cell Counting
  The ability to accurately determine cell number is an important aspect of a broad range of applications, including setting up and optimizing cell-based assays, normalizing data across samples, and conducting cell proliferation assays.
  
  The Agilent BioTek Lionheart FX automated microscope and Agilent BioTek Cytation cell imaging multi-mode readers utilize label-free and fluorescence imaging modes, along with powerful Agilent BioTek Gen5 image analysis tools, to deliver efficient and effective automated cell counting.
- Cancer Research
  The field of cancer research spans diverse disciplines and requires a multipronged approach to understand the complex factors driving cancer formation and progression, as well as for the development of effective therapeutics.
  
  BioTek instruments provide versatile platforms and integrated solutions for conducting a broad range of cancer research studies.
- Cell Culture QC
  
  Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points.
- Cell Health and Viability
  
  Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury.
- Cell Metabolism
  
  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
- 细胞侵袭/迁移
  
  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
- Developmental Biology
  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Endotoxin Analysis
  Bacterial endotoxins are lipopolysaccharides (LPS) generally found in the outer membrane of gram-negative bacteria, such as E.coli, and are widely present in our environment. Because of the pyrogenic nature of endotoxins, with human effects from fever to hemorrhagic stroke, FDA regulations require that raw materials (e.g. water) used in parenteral drugs be tested for endotoxin levels.
- Histology and Slide Scanning
  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation™ and Lionheart™ FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5™ image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
- 微生物
  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
- Molecular Biology and Biochemical
  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
技术资源
- 应用手册
  
  BioTek 的应用手册为用户展现了最尖端的科研内容，覆盖产品线最为广泛的应用，可以通过标题，检测，产品或简单的关键词来进行应用搜索，从而获取您感兴趣的应用手册。
- 动态摘要
  
  BioTek’s application bulletins provide brief, concise descriptions of timely applications enabled with BioTek instrumentation.
- 应用指南
  
  很多应用是简单明确无需解释的，但是很多复杂实验和流程的操作方法和操作要点则对实验是最为有用的。BioTek的应用指南会为不同的应用主题提供相关操作要点.
- 期刊论文
  搜索浏览我们的数据库查找科学文章获取BioTek在顶级杂质上所发表的科学应用文章。
- 科学海报
  BioTek的应用团队已经退出超过170篇科学海报均为我们相关产品所涉及的最新科学应用热点。浏览或搜索主题或感兴趣的产品，获取相关海报。
- 技术说明
  
  Our Technical Notes illustrate product-specific capabilities and common applications for BioTek microplate instrumentation, and their implementation in common experimental design.
- Visual Abstracts
  BioTek’s Visual Abstracts are brief animated presentations describing the workflow of a single specific imaging or multi-mode detection application.
- 网络视频
  随时观看我们的视频活动可通过主题搜索，应用名称或产品名称来搜索感兴趣的视频，也可以注册邀请函，参加近期视频 .
- 白皮书
  浏览BioTek的白皮书及时获得重要的内容如活性氧族和3D细胞培养。
- 引用文献
  
  您可以在我们的数据库中搜索我们产品超过3000篇以上的科学引用文献，关注全球用户的使用情况。
- 视频
  Here you will find a collection of videos found throughout our site. These videos can also be found on BioTek's YouTube channel.
- 近期讲座
  通过我们的网络讲座，BioTek始终坚持与用户分享最新的应用信息和资源。今天注册并查看近期您关注的讲座主题。
服务与支持
- 销售及支援办事处
  BioTek高素质的销售和技术专家，均是生命科学设备的专业人员，可以知道您通过您的诉求帮您选择合适的设备，并辅助您安装和培训人员。
- 技术支持
  BioTek 的全球技术支持中心 (GTS)是一个帮助平台，回答有关BioTek产品的使用，维护，故障解决等常见问题。通常GTS为无假日服务，提供处假期外的全周咨询服务。每天的服务时间为：美国东部标准时间 8:30 A.M. - 5:30 P.M。
- 全球服务
  BioTek通过我们全球范围的几个服务中心，为同于提供仪器服务和维修。区域维修人员可以为大多数用户提供全球范围内的服务。
- 常见问题
  通过关键词，产品或目录搜索常见问题。如需更多帮助请拨打(800) 242-4685 或通过我们的问题调查表提交您的问题。
- 客户资源中心
  BioTek的客户资源中心(CRC) 为客户提供一个窗口，可以查询相关产品和软件等信息。注册和登录：
  
  - 检查状态和订单追踪
  - 管理设备目录
  - 获取文件，用户手册和软件
  - 寻求服务&支持
- 需要支持
  Contact BioTek today. Ask a sales or technical question or request service.
关于我们
- 新闻
  Synergy Neo2 and Synergy H1 Now Available with Expanded Temperature Control Range
  July 28, 2021 - Now with temperature control up to 70 ºC, BioTek’s Synergy Neo2 Hybrid Multi-Mode Reader and Synergy H1 Hybrid Multi-Mode Reader aid in more assay workflows than ever before. Application flexibility extends to enzyme kinetics, protein stability and aggregation, and projects incorporating loop mediated isothermal amplification (LAMP) assays. 浏览更多
  
  BioTek's Cytation 7 Cell Imaging Multi-Mode Reader Named ‘Best New Drug Discovery & Development Product’ at Scientists’ Choice Awards
  May 5, 2021 - BioTek Instruments is pleased to announce that the Cytation 7 Cell Imaging Multi-Mode Reader was awarded ‘Best New Drug Discovery & Development Product’ of 2020. The honor was voted upon by SelectScience members across the world and celebrated at the 2021 Scientists’ Choice Awards online awards ceremony. 浏览更多
  
  BioTek Announces New Cytation C10 Confocal Imaging Reader
  February 2, 2021 - BioTek Instruments, a part of Agilent, is excited to unveil the Cytation C10, an advanced, automated Confocal Imaging Reader. This versatile and multi-functional automated system is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and quantitative data to support cell-based research. 浏览更多
  更多新闻
- 客户聚焦
  We are dedicated to developing and supporting instrumentation that enables a broad range of applications for scientists around the world. We can tell you about how BioTek instrumentation can benefit your research, but we’d rather you hear it directly from our customers! Browse the Customer Spotlights below to see how BioTek is helping to make their research possible.
- 活动
  LS2 Annual Meeting 2022
  
  February 16 - November 18, 2022
  
  Booth #21
  
  Irchel campus, University of Zurich
  BioTek instrumentation will be on display by BioTek Switzerland.
  
  Zurich
  Switzerland
  
  Experimental Biology 2022
  
  April 2 - November 5, 2022
  
  Booth #605
  
  Pennsylvania Convention Center
  BioTek instrumentation will be on display by BioTek U.S.
  
  Philadelphia. PA
  United States
  
  更多事件
- 获奖
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- 历史
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
- 合作伙伴
  作为我们非常重视的合作伙伴，BioTek可以通过产品、服务，支持，培训，质量保证、市场共赢等方式帮助您产品的销售、市场拓展及核心业务发展，并满足您客户的需求
- 微博
  关注我们的微博, 科学讲堂,关注我们的科学家和仪器专家的内容分享。
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- Quality and Compliance
  BioTek is committed to maintaining the high quality of our products and services. All of our products are designed and manufactured in the USA at our Headquarters in Winooski, Vermont. Our continual improvement process includes soliciting customer input on quality and product features, and then designing, validating, manufacturing, and testing products to ensure performance and reliability.
- 人才招聘
  在BioTek我们热切而真诚的将员工看成是我们最重要的财富。我们保持高速的团队建设，最近5年全球雇员人数增加了一倍。在美国佛蒙特州美丽的绿山脚下，坐落着我们的全球总部，并设有支持全球区域的办公室。
  
  如果你有兴趣成为我们神奇团队的一员，请查看我们今天的工作岗位一览表。
- 联系我们
  Contact BioTek today. Ask a sales or technical question or request service.

SEARCH

The enzyme linked immunosorbent assay (ELISA) is a very common, robust technique for detecting various analytes. It spawned a broad range of immunoassays that utilize either wash or no-wash workflows using colorimetric, fluorometric, luminometric, TRF or TR-FRET, AlphaScreen and AlphaLISA detection technologies. Some of these latter technologies utilize low volume analysis in 1536-well plates, yet ELISA performed in 96-well microplates remains the most used immunoassay technique. The ELISA technique causes formation of specific immune complexes that are easily measured using the Agilent BioTek single or multimode microplate readers. The basic building blocks of an ELISA reaction are:

ELISA process

Application Notes

Technical Notes

Benchtop ELISA Automation with BenchCel Microplate Handler

Publications

Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination

Application Bulletins

Detection of HTLV I and II Antibodies using Absorbance based ELISA

Learn more about ELISA – see our applications and technical documentation in Resources.

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For Research Use Only. Not for use in diagnostic procedures.

RA44216.3566203704