ELISA

The Enzyme Linked Immunosorbent Assay (ELISA) is a very common, robust technique for detecting various analytes. It spawned a broad range of immunoassays that utilize either wash or no-wash workflows using colorimetric, fluorometric, luminometric, TRF or TR-FRET, AlphaScreen and AlphaLISA detection technologies. Some of these latter technologies utilize low volume analysis in 1536-well plates, yet ELISA performed in 96-well microplates remains the most commonly used immunoassay technique. The ELISA technique causes formation of specific immune complexes that are easily measured using BioTek’s single or multi-mode microplate readers. The basic “building blocks” of an ELISA reaction are:

Application Notes

• Versatile, Cost-Effective Automation of Avian Influenza and Mycoplasma Gallispeticum-Synovaie ELISAs
• Automated ELISA Liquid Handling with the EL406™ Combination Washer Dispenser - Semi-Automation of ELISA Process
• Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis
• Concentration and Detection of Low Levels of Escherichia coli O157:H7, Listeria monocytogenes 4b, and Salmonella enterica Typhimurium in High Organic Load Lettuce Wash
• Using EL406™ Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation
• Histamine Analysis in Wine Samples Using the Microplate Format
• AlphaLISA: Human Insulin Quantification Using Synergy 4 Hybrid Multi-Mode Microplate Reader

Technical Notes

• Benchtop ELISA Automation with BenchCel Microplate Handler

Publications

• Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination

Application Bulletins

• Detection of HTLV I and II Antibodies using Absorbance based ELISA

Learn more about ELISA  – see our Applications and technical documentation in Resources.

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• Epoch 2 Microplate Spectrophotometer
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• EL406 Washer Dispenser
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