ELISA

The enzyme linked immunosorbent assay (ELISA) is a very common, robust technique for detecting various analytes. It spawned a broad range of immunoassays that utilize either wash or no-wash workflows using colorimetric, fluorometric, luminometric, TRF or TR-FRET, AlphaScreen and AlphaLISA detection technologies. Some of these latter technologies utilize low volume analysis in 1536-well plates, yet ELISA performed in 96-well microplates remains the most used immunoassay technique. The ELISA technique causes formation of specific immune complexes that are easily measured using the Agilent BioTek single or multimode microplate readers. The basic building blocks of an ELISA reaction are:

Application Notes

• Versatile, Cost-Effective Automation of Avian Influenza and Mycoplasma Gallispeticum-Synovaie ELISAs
• Automated ELISA Liquid Handling with the EL406™ Combination Washer Dispenser - Semi-Automation of ELISA Process
• Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis
• Concentration and Detection of Low Levels of Escherichia coli O157:H7, Listeria monocytogenes 4b, and Salmonella enterica Typhimurium in High Organic Load Lettuce Wash
• Using EL406™ Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation
• Histamine Analysis in Wine Samples Using the Microplate Format
• AlphaLISA: Human Insulin Quantification Using Synergy 4 Hybrid Multi-Mode Microplate Reader

Technical Notes

• Benchtop ELISA Automation with BenchCel Microplate Handler

Publications

• Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination

Application Bulletins

• Detection of HTLV I and II Antibodies using Absorbance based ELISA

Learn more about ELISA  – see our applications and technical documentation in Resources.

Related Products:

• Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader
• Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader
• Agilent BioTek Cytation 1 Cell Imaging Multi-Mode Reader
• Agilent BioTek Synergy Neo2 Hybrid Multi-Mode Reader
• Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader
• Agilent BioTek Synergy HTX Multi-Mode Reader
• Agilent BioTek Synergy LX Multi-Mode Reader
• Agilent BioTek Epoch 2 Microplate Spectrophotometer
• Agilent BioTek Epoch Microplate Spectrophotometer
• Agilent BioTek ELx808 Absorbance Reader
• Agilent BioTek 800 TS Absorbance Reader
• Agilent BioTek EL406 Washer Dispenser
• Agilent BioTek 405 TS Washer
• Agilent BioTek 405 LS Washer
• Agilent BioTek 50 TS Washer
• Agilent BioTek MultiFlo FX Multi-Mode Dispenser

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For Research Use Only. Not for use in diagnostic procedures.

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