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What are the commonly used ELISA conjugate/substrate combinations and what wavelengths do they require?
Enzyme/Substrate Systems for ELISA
|
|
|
Reading wavelength (nm) |
|
|
Enzyme label |
System |
Nonstopped |
Stopped |
|
Horseradish peroxidase (HRP) |
OPD |
450 |
492 |
|
|
TMB |
630 |
450 |
|
|
ABTS |
414 |
414 |
|
|
5AS |
450 |
450 |
|
Alkaline Phosphatase (Alk-phos) |
pnpp |
405 |
405 |
|
β-Galactosidase (β-gal) |
ONPG |
420 |
420 |
|
Urease |
Urea bromocresol |
588 |
588 |
