SynergyH1 全功能微孔板检测仪
Synergy™ H1 is a flexible monochromator-based multi-mode microplate reader that can be turned into a high-performance hybrid system with the addition of a filter-based optical module. The monochromator optics uses a third generation quadruple grating design that allows working at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate applications found in life science research laboratories.
The filter module is a completely independent add-on that includes its own light source, and a high performance dichroic-based wavelength selection system. With its very high optical efficiency, this module supports advanced detection modes such as Fluorescence Polarization, Time-Resolved Fluorescence & TR-FRET and filtered luminescence (e.g. BRET). A dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).
To create the ideal physiological environment for live-cell assays, the new Gas Controller for Synergy H1 allows control and monitoring of CO2 and O2 levels within the Synergy H1. The Gas Controller, along with user-adjustable orbital shaking and advanced 4-ZoneTM temperature control make up the CellControlTM feature set available for the Synergy H1 readers.
HTRF® and the HTRF logo are registered trademarks of Cisbio.
- Patent-Pending Hybrid Technology combines flexible monochromator detection with high performance dichroic-based detection.
- Compatible with the Gas Controller for control and monitoring of CO2 and O2.
- Compatible with Take3™ Multi-Volume Plate: samples down to 2 µL volume can be measured. Especially useful when working with precious samples, for fast and accurate DNA/RNA quantification at 260 nm
- Quadruple grating monochromator for maximum flexibility and ease of use.
- Dichroic-based filter optics, for best performance and advanced detection technologies such as fluorescence polarization and time resolved fluorescence.
- Comes with Gen5 software: reader control, advanced data analysis and flexible Excel export in one software package.
| Model | Synergy H1 | |||||
| Part # | H1M | H1MD | H1F | H1FD | H1MF | H1MFD |
| Monochromator fluorescence | • | • | • | • | ||
| Monochromator absorbance | • | • | • | • | ||
| Full-light luminescence | • | • | • | • | • | • |
| Filter/dichroic fluorescence | • | • | • | • | ||
| Fluorescence polarization | • | • | • | • | ||
| Time resolved fluorescence | • * | • * | • | • | • | • |
| Filtered luminescence | • | • | • | • | ||
| Dual reagent dispenser | • | • | • | |||
| Temperature control to 45°C | • | • | • | • | • | • |
| Gen5 data analysis software | • | • | • | • | • | • |
| * Secondary mode | ||||||
| Gas Controller Ready Models** | H1MG | H1MDG | H1FG | H1FDG | H1FMG | H1MFDG |
| ** These models have the same features as the non- Gas Controller Ready models in the chart above. Gas Controller sold separately. | ||||||
| 常规 | |
| 波长选择 | Hybrid Technology™ (专利申请中) 四光栅和滤光片/ 二向色镜 |
| 检测手段 | 光栅系统：荧光，化学发光，UV-可见吸收光 滤光片系统：荧光，时间分辨荧光，荧光偏振，发光 |
| 读板方法 | 终点法，动力学法，光谱扫描，孔域扫描 |
| 微孔板类型 | 1-384孔板 兼容Take3 板进行2 µL 微量样品检测 |
| 温度控制 | 至45°C, ±0.5 °C @ 37°C |
| 震荡 | 可以 |
| 软件 | Gen5™ 仪器控制,高级数据分析, Microsoft® Excel® 导出. 可通过USB 或串口进行连接。 |
| 自动化 | 可以 |
| CO2 和 O2 控制 | SynergyH1可选气体控制系统 |
| 吸光度 | |
| 光源 | 氙闪灯 |
| 波长选择 | 单色器 |
| 波长范围 | 230-999nm，1nm步进 |
| 带通 | 4 nm (230-285 nm), 8 nm (>285 nm) |
| 动态范围 | 0 - 4.0 OD |
| 分辨率 | 0.0001 OD |
| 光程校正 | 可以 |
| 单色器波长准确性 | ±2 nm |
| 单色器波长可重复性 | ±0.2 nm |
| OD 准确性 | < 1% @ 2.0 OD < 3% @ 3.0 OD |
| OD 线性 | < 1% 0 -3.0 OD |
| OD 可重复性 | < 0.5% @ 2.0 OD |
| 散射光 | 0.03% @ 230 nm |
| 读板速度 | 96: 11 秒 384: 22 秒 |
| 荧光强度 | |
| 灵敏度 | 光栅系统: 顶部荧光素： 2.5 pM (0.25 fmol/孔 384-孔板) 底部荧光素：5 pM (0.5 fmol/孔 384-孔板)
荧光素 1 pM (0.1 fmol/孔 384-孔板) |
| 光源 | 氙闪灯 |
| 波长选择 | 双光栅单色器（顶/底部）以及深度阻挡带通滤光片/二向色镜（顶部） |
| 波长范围 | 200 - 700 nm (900 nm 可选) |
| 动态范围 | 5个数量级 |
| 检测系统 | 两个PMT检测器：一个用于单色器系统，一个用于滤光片系统 |
| 读板速度 | 96: 11 秒 384: 22 秒 |
| 化学发光 | |
| 灵敏度 | 单色器系统: 20 amol ATP (闪光) 滤光片系统: 10 amol ATP (闪光) |
| 波长范围 | 300 - 700 nm |
| 动态范围 | > 6 个数量级 |
| 荧光偏振 | |
| 灵敏度 | 5 mP @1 nM 荧光素 |
| 波长范围 | 400- 700 nm (300-900 nm 可选) |
| 时间分辨荧光 | |
| 光源 | 氙闪灯 |
| 灵敏度 | Europium 100 fM 滤光片系统 (10 amol/孔 384-孔板) |
| 波长范围 | 200 - 700 nm (900 nm 可选) |
| 试剂分液器 | |
| 分液精度 | ≤2% @ 50-200 µL |
| 分液准确性 | ±1 µL 或 2% |
| 数量 | 2个注射器泵 |
| 孔板制式 | 1- 至 384-孔微孔板 |
| 分液容量 | 5 - 1000 µl 1 µl 步进 |
| 死体积 | 1 mL, 100 µL 回流 |
| 物理特性 | |
| 功率 | 130 瓦特 最高 |
| 尺寸 | 15.4"长 18.6"宽 12.9"高 (39.1 x 47.2 x 32.8 cm) |
| 重量 | 50 磅 (22.5 kg) |
| 法规 | |
| 法规 | 所有BioTek微孔板设备均符合CE和ETL安全标志认证。需要获得认证详细信息请联系BioTek。 |
具体参数可能会进行调整。
标准配置（包括）
Gen5 数据分析软件
可选
BioStack 微孔板储板器
