BioTek: Microplate Instrumentation & Software Solutions
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405 Touch 微孔板洗板机

The new 405™ Touch Microplate Washer incorporates a state-of-the-art, high resolution LED backlit touch screen user interface for intuitive and flexible onboard instrument control of 96- and 384-well microplate washing. A 5.7 inch graphical color display seamlessly guides users through protocol development, instrument maintenance and daily use at the touch of a finger. Two USB flash drive ports enable convenient file transfer, storage and operation. For those who prefer computer control or require 21 CFR Part 11 compliance, the 405 Touch can be controlled with optional Liquid Handling Control™ Software. 

For over 27 years, BioTek has continued to advance the art of automated microplate washing, resulting in the industry leading ELx405™, which set the standard in full 96- and 384-well washing.  The 405 Touch, maintains all the features of the ELx405™ Microplate Washer while adding a new,  modern and powerful interface to again reshape the day-to-day routines of today’s life science laboratories.  Fast and efficient vacuum filtration, high strength biomagnetic separation, patented Dual-Action™ manifolds, optimized cell washing for loosely adherent cell monolayers and patent-pending Ultrasonic Advantage™ are all available.  New key features include automated internal 4-buffer switching and quick release manifolds for easy maintenance and modular use.

Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. BioTek’s washers with their built-in ultrasonic cleaners are the only instruments of their kind available today able to automatically prime with cleaning solution, sonicate manifold tubes to remove protein or salt crystal build up and re-prime with buffer leaving the instrument clean and ready for its next wash. Cleaning these tubes is a time consuming and labor intensive process. BioTek's Ultrasonic Advantage™ incorporates the power of ultrasonic cleaning to make the 405 Touch a self-maintaining microplate washer.

For washing microsphere based assays, all 405 Touch models can be equipped with accessory modules for both vacuum filtration and biomagnetic separation. This allows for full plate washing of polystyrene and magnetic beads used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex® xMAP® technology platform. The 405 Touch’s vacuum filtration feature automates the wash steps of filter bottom plates with five different vacuum levels for optimal performance of filter pore sizes ranging from 0.45 µm to 1.2 µm. Developed with leaders in genotyping, gene expression and protein assays, the 405 Touch’s biomagnetic separation incorporates a range of immobilization options each with high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention. The 405 Touch is Luminex® xMAP® approved.

The 405 Touch Washer, also known as the 405 TS Series, is available in a range of configurations for different microplate formats and wash requirements.  The 405 HT TS is designed specifically to rapidly wash 384-well microplates and includes BioTek's patented Dual-Action™ 192-tube manifold to ensure optimal performance. A proprietary tube-in-tube aspiration manifold design also provides complete 384-well evacuation even with buffers not containing surfactant.  The 405 Select TS offers the patented Dual-Action™ manifold in a 96-tube format, providing independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection. This unique design washes both 96- and 384-well plates without the need for any hardware changes. With its angled dispense tubes, full control of dispense and aspiration rates and unique X, Y, Z positioning, the 405 Select TS is ideal for the most demanding wash requirements including loosely-adherent cell lines. For those applications that use only 96-well microplates, the standard 405 TS is an ideal choice with unique features to lower background absorbance values and increase the signal-to-noise ratio of typical ELISA assays.

With its color graphical user interface and wide application range, the new 405 Touch Microplate Washer is an easy to use, total solution for 96- and 384-well plate washing in a compact and robot-compatible design. 

Applications:

  • ELISAs
  • MSD assays
  • HCS immune cytochemistry
  • FLIPR® Ca2+ flux
  • Cell-based assays
  • Magnetic and polystyrene bead processing (gene expression assays, cytokine assays)
  • ELISPOT assays

US Federal Government customers: This product is available on GSA contract. Contact BioTek Customer Care with questions or submit a Quote Request Form to receive pricing and information on specific models (please note 'GSA Contract' in the Comments field).

 

xMAP® is a registered trademark of Luminex® Corporation.
FLIPR® is a registered trademark of Molecular Devices®

Hardware:

  • Accommodates 96- and 384-well microplates
  • High resolution, LED backlit touch screen UI for intuitive and flexible onboard washer control
  • 5.7 inch graphical color display seamlessly guides users through protocol creation, instrument maintenance and daily use at the touch of a finger
  • Fast Intel Atom processor running Windows CE
  • Resistive touch screen display

    - Supports all gloved environments
    - Compatible with widest range in humidity levels
    - May be operated with pointing devices
    - Low power, green technology

  • Two USB flash drive ports enable convenient file transfer, storage and operation

    - Especially helpful when instrument is shared between researchers
    - Insert memory stick, wash plates…and go
    - Each unit ships with memory stick containing sample protocols and instructional videos

  • 405 HT TS

    - Offers fast 384-well washing with patented 192-tube Dual-Action™ manifold for independent dispense and aspiration control for precise overfill washing and overflow protection

  • - Includes patent-pending Ultrasonic Advantage™ – built in ultrasonic cleaner for manifold self-maintenance
    - Equipped with superior tube-in-tube aspiration manifold design for 384-well washing with buffers not containing surfactant
    - Also calibrated for accessory 96-tube Dual-Action manifold for 96-well washing

  • 405 Select TS

    - Accommodates 96-/384-well washing with patented 96-tube Dual-Action™ manifold
    - Includes optimized cell washing with two low-flow rates and 20° angled dispense tubes ideal for washing loosely adherent cell monolayers

  • 405 TS models automate basic 96-well dispensing and aspiration
  • Automated internal 4-buffer switching
  • Internal positive displacement pump eliminates use of troublesome pressure delivery systems and allows use of any size dispense reservoir
  • Inline filtration within each dispense bottle
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates need for blank plates when used in automated robotic systems
  • All washers vacuum filtration ready (accessory filtration modules sold separately)
  • All washers biomagnetic separation ready (accessory magnets sold separately)
  • Quick change manifold designs
  • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
  • BioStack™ Microplate Stacker compatibility for batch processing of up to 50 ANSI/SBS standard height plates

Software:

  • Context sensitive help system complete with recommendations, diagrams and images is only a key press away when questions arise
  • Instructional videos walk users through instrument setup and basic use
  • Quick menu allows fluid paths to be easily primed, wash process started and manifold cleaned
  • Predefined protocols included for out-of-box washing

    - ELISA
    - Cell washing
    - Vacuum filtration and biomagnetic separation – Luminex® xMAP® technology workflows

  • Fully customizable protocol parameters for individualized wash requirements - gentle cell-washing to vigorous wash for high-binding assays

    - Dispense height
    - Flow rate
    - Aspiration height
    - Soak time
    - Shake time and frequency
    - Aspiration rate
    - Horizontal dispense and aspirate positions
    - Crosswise aspiration

  • AutoPrime
  • Additional software flexibility and 21 CFR Part 11 compliance with Liquid Handling Control™(LHC) Software for method development, downloading and washing plates.
  • Run protocols created onboard, downloaded from LHC™ Software or transferred on USB drive
型号
货号#
96-/384-孔板
384-孔板
96-孔板
细胞清洗
4种缓冲液切换
超声清洗
磁板清洗*
滤板清洗*
405™ HT TS
405TSHT
 
    
405TSHTV
 
  
405™ Select TS
405TSU
   
  
405TSUV
 
 
 
405TSUS
  
 
405TSUVS
  
405™ TS
405TSR
 
 
 
 
 
405TSRV
   
 
 
405TSRS
  
 
 
405TSRVS
  
 

 

* 需额外购买配件 

405™ Touch 微孔板洗板机配置
 特点设计
HT快速 384-孔微孔板清洗
  • 192-道 (16 x 12) 洗头
  • Dual-Action™
V自动4种缓冲液切换 
SUltrasonic Advantage™
  • 内置超声清洗模块,进行自我维护
U96-/384-孔板清洗
  • 96-道 (8 x 12)洗头
  • Dual-Action™
R96-孔板清洗
  • 96-道(8 x 12) 洗头

 

 

常规
分析 ELISA
基于细胞的分析(依型号而定)
磁珠, 聚乙烯微球
  • 多重分析
  • 基于微球的ELISA

废液抽滤处理
分离 生物磁性分离,真空抽滤
微孔板类型 96-和384-孔 (依型号而定)
浅孔和标准高度板
实心和滤底板
  • 滤膜直径0.45 µm 至1.2 µm
震荡 程序设定分钟、秒,最高达60分钟 强度-低,中,高或可变
浸泡时间 程序设定分钟、秒,最高达60分钟
磁力 高强度96和384孔板制式
洗板机用户可根据需要选择两种不同的磁板设计:
平面磁板:
- 平底板- 微球在板底成带状吸附
-圆底板-微球在板底成团状吸附
环状磁板: - 微球在板底成四角吸附
安全性/便捷/维护 废液和真空感应,流量和流速测定 气溶胶防护罩 溢出保护 预置维护程序
自动化 BioStack™微孔板储板器- 最多了处理 50块符合 ANSI/SBS标准高度的微孔板 (选配)
  • 不包括滤底板
机载软件 高清晰背光LED触摸式操作屏 5.7英寸彩色数字显示 Itel Atom 处理器,Windows CE 预置模板程序-ELISA,细胞清洗,磁珠清洗,生物磁性分离和真空抽滤 快速菜单 创建和编辑多重用户自定义复杂程序 运行面板程序、LHC™软件下载程序或USB闪存存贮程序
PC 软件 Liquid Handling Control™ (LHC™), 用于习惯于PC操作环境的用户进行程序编辑和系统控制(选配)
操作温度 15° C 至30° C (59° F 至 86° F)
接口 PC 控制和BioStack™ 整合 – 1 个USB 串口, 1个 9-针 RS232 串口
For file transfer – 2 USB Flash Memory ports
湿度 10% 至 85%, 无凝集
电的 100 – 240 Volts AC 50/60 Hz, < 8.0 A
清洗
洗头类型 96-孔板清洗:
96-道 (8 X 12) 洗头
96-/384-孔板清洗:
Dual-Action™ 96-道 (8 X 12) 洗头
384-孔板清洗:
Dual-Action™ 192-道(16 x 12) 洗头
清洗速度 实心底板:

96-孔:
3 抽吸 / 分液.循环, 300 µL/孔, 96-道 洗头, 最后抽吸: <30 秒
384-孔:
3 抽吸. / 分液. 循环, 100 µL/孔, 96-道洗头, 最后抽吸: <80 秒
1 抽吸. / 分液. 循环, 400 µL/孔, 384 孔, 192-道洗头, 最后抽吸: <20 秒


滤底板:
可变, 依不同程序而定
液体输送 内置正压可置换泵
真空抽滤 可选真空抽滤水平(30秒,接近完成):

最低: -38 mmHg
低: -113 mmHg
中: -200 mmHg
高: -390 mmHg
最高: -506 mmHg

真空抽滤时间:
5 至 999 秒
容量范围 25 3,000 µL/孔
可选1 µL 递进
洗液选择 内置4洗液自动切换(可选)
流速 1 至 11
包括低流速, 细胞清洗保护流速(依型号而定)
清洗循环 1 至 250
分液精确度
  • 96-孔洗板- 96-道 (8 x 12) 洗头:
    3% CV: 300 µL/孔(去离子水含0.1% Tween 20 和 FD&C #1 蓝色染料), 速率 6, 630 nm下读取吸收光值,  450 nm 做为参考波长
     
  • 384-孔板清晰- 192-道(16 x 12) 洗头:
    < 4% CV: 80 µL/孔(去离子水含0.1% Tween 20 和 FD&C #1 蓝色染料), 速率 7, 630 nm下读取吸收光值,  450 nm 做为参考波长
残留量 实心底板:
  • 96-孔清洗- 96-道 (8 x 12) 洗头:
2 µL/孔: 3-循环清洗, 300 µL/孔 (去离子水加0.1% Tween 20), 康宁96-孔平地板, 检测前优化抽吸高度
  • 384-孔板清洗- 192-道 (16 x 12) 洗头:
< 2 µL/孔: 3-清洗循环, 100 µL/孔(去离子水加0.1% Tween 20), 康宁96-孔平底板, 测试前优化抽吸高度

滤底板:
  • 96-孔:
平均每板增加重量< 1.2 g 分液300 µL 至
0.45 µm 板, 抽滤30 秒, 低真空,堵塞
  • 384-孔:
平均每板增重 < 4.0 g 分液80 µL 至 1.2
µm 板, 抽滤 10 秒, 低真空度, 堵塞
灭菌 化学消毒 0.5%次氯酸钠,70%异丙醇或70%乙醇
超声波清洗 内置超声清洗功能清除蛋白和盐结晶,程序定义小时,分钟最高60分钟(依型号而定)
供料瓶容量 4 L 或 10 L (可选)
物理特性
尺寸 长: 17" (43.2 cm)
宽: 14" (35.6 cm)
高: 10" (25.4 cm)
重量 带内置洗液切换模块 - 36 lbs (16.5 kg)
不带 - 30 lbs (13.5 kg)
法规
法规 仅用于体外诊断。所有BioTek微孔板仪器均符合CE和TUV认证

具体参数可能会进行调整。

可选

BioStack 微孔板储板器

BioStack 微孔板储板器

BioStack™ Microplate Stacker adds stacking capabilities to BioTek's Synergy™, PowerWave™, 405™, ELx405™ Select Deep Well, EL406™, MultiFlo™ and Precision™ product families.


Liquid Handling Control 软件

Liquid Handling Control 软件

Software is a powerful yet flexible interface for use with BioTek’s liquid handlers including the 405™ Microplate Washer, the ELx405™ Select Deep Well Washer, the EL406™ Washer Dispenser and the MultiFlo™ Dispenser. New LHC Secure offers compliance to the FDA directive 21 CFR Part 11 or whenever additional security is required.


标准配置(包括)

操作手册

MANUAL Operator's manuals can be obtained via the Customer Resource Center

可选

BioStack 集成工具包

7310035 BioStack Integration Kit for 405™ TS and 405™ LS

瓶子和管道

1173000 Dispense Bottle - with Filter, 10L
1173002 Cap for Dispense Bottle - with Filter, 10L
1173003 Cap for Dispense Bottle - with Filter, 4L
1173031 Dispense Bottle – with Filter, 4L
7100531 Cap for Waste Bottle, 4L (Also fits 10L or 20L) 带有图片的配件
7100533 Waste Tubing Set 带有图片的配件
7100534 Waste Bottle, 4L 带有图片的配件
7100537 Dispense Tubing Set – 4 Buffers
7100538 Dispense Tubing Set –1 Buffer 带有图片的配件
7100542 Waste Bottle with Level Sensing, 4L 带有图片的配件
7100543 Waste Set with Level Sensing, 4L Bottles 带有图片的配件
7100544 Cap for Waste Bottle with Level Sensing, 4L 带有图片的配件
7100556 Waste Bottle and Tubing, 20L 带有图片的配件
7100557 Waste Bottle and Tubing, 10L 带有图片的配件

分液/废液系统

1170528 Complete Dispense System, 10L Bottles
1170529 Complete Dispense System, 4L Bottles
1170530 Complete Dispense/Waste System 115V/230V, 4L Bottles
1170531 Complete High Flow Waste System 115V/230V, 10L Bottles
1170532 Complete Dispense/High Flow Waste System 115V/230V, 4L Bottles
1170533 Complete Dispense/High Flow Waste System 115V/230V, 10L Bottles
1170534 Complete Waste System 115V/230V, 10L Bottles
1170535 Complete Dispense/Waste System 115V/230V, 10L Bottles
1170536 Complete Dispense/Direct Drain Waste System 115V/230V, 4L Bottles
1170537 Complete Dispense/Direct Drain Waste System 115V/230V, 10L Bottles
7100582 Vacuum Set with Level Sense Assembly, 10L 带有图片的配件
7100746 Complete Waste System 115V/230V, 4L Bottles 带有图片的配件
7100753 Complete High Flow Waste System 115V/230V, 4L Bottles
7103024 Standard Vacuum Pump 115V/230V 带有图片的配件

滤光片

01310 Filter for Dispense Bottle
48294 Filter for Waste Tubing 带有图片的配件

岐管

1170010 96-tube Dual-Action™ Manifold Kit – with 7° Dispense Manifold and Tube - in - Tube Aspiration Manifold
1172046S 96-tube Dual-Action™ Tube-in-Tube Aspiration Manifold
7102068S 96-tube Dual-Action™ 7° Dispense Manifold

其他

01113 Silencing Muffler for Standard Vacuum Pump
1170008 96-well Vacuum Filtration Kit
1170009 96-/384-well Vacuum Filtration Kit
4030551 Vacuum Gauge/Regulator
7100025 Stylus Kit for any 405™/ELx405™ Select Deep Well manifold 带有图片的配件
7102215 384-well Ring Magnet 带有图片的配件
7102216 96-well Ring Magnet 带有图片的配件
7103016 96-well Flat Magnet 带有图片的配件
7103017 384-well Flat Magnet 带有图片的配件

产品认证套包

1170522 405™ TS Qualification Package - IQ/OQ/PQ package on CD

询价